SFB 1208: Identity and Dynamics of Membrane Systems - from Molecules to Cellular Functions

生物膜在单细胞内外空间、亚细胞隔间以及多细胞生物体的细胞间保持非平衡状态。生命的先决条件是膜不是静态的实体，而是由不断变化的边界组成，这些边界对内部和外部的提示做出反应。膜如何获得其特性并控制其时空动力学？它们的组成成分扮演着什么角色，主要是不同种类的膜蛋白和蛋白复合体？这些都是将在《儿童权利公约》中讨论的基本问题。作为模型系统，我们选择了不同的单细胞和多细胞生物来研究它们的膜系统的特性和动力学。我们的研究将继续包括双管齐下的方法，从单个膜蛋白及其复合体的水平以及整个细胞膜系统开始。我们在我们的多方面联合体中结合了一系列独特的结构、生化和细胞生物技术，旨在揭示从纳秒到几天的整个生物相关时间尺度内的分子和细胞过程。我们的多学科方法，包括在方法和概念层面的广泛合作，旨在破译不同膜系统的组件和膜本身之间的多维相互作用，最终形成丰富的生物过程的基础。

Biological membranes maintain a non-equilibrium state between the inside and outside space of single cells, subcellular compartments, and between the cells of multicellular organisms. A prerequisite for life is that membranes are not static entities but comprise constantly changing boundaries that react in response to internal and external cues. How do membranes obtain their identity and control their spatio-temporal dynamics? Which role do their constituents play, mainly the different kinds of membrane proteins and protein complexes? These are the fundamental questions that will be addressed within this CRC. As model systems, we have chosen different uni- and multicellular organisms to investigate identity and dynamics of their membrane systems. Our studies will continue to comprise a two-pronged approach, starting from both, the level of individual membrane proteins and their complexes as well as entire cellular membrane systems. We combine in our multifaceted consortium a unique range of structural, biochemical and cell biological technologies, which aims to unravel the molecular and cellular processes within the entire biological relevant timescale from nanoseconds to days. Our multidisciplinary approach, which involves extensive collaborations at the methodological and conceptual level, aims to decipher the multidimensional interplay between components of different membrane systems and the membranes themselves that ultimately form the basis for a wealth of biological processes.