Establishment of endosomal membrane domains by guanine nucleotide exchange factors (P17)

项目P17研究了鸟嘌呤核苷酸交换因子(GEF)如何整合不同的细胞线索，以适当地激活它们在细胞内交通的时空协调中的同源Rab GTP酶。该小组将通过(I)调节蛋白的生化和结构特征，(Ii)通过体外脂类相互作用和体内亚细胞定位研究促黄体生成素-促黄体生成素的膜结合，以及(Iii)确定促黄体生成素-促黄体生成素的原子结构和整体结构，阐明特定类型的促黄体生成素-促黄体生成素如何建立微隔室的分子基础。

Project P17 investigates how guanine nucleotide exchange factors (GEFs) integrate different cellular cues to properly activate their cognate Rab GTPase in the spatio-temporal coordination of intracellular traffic. The group will elucidate the molecular basis how a particular class of GEFs, LH-GEFs, establish microcompartments by (i) biochemical and structural characterization of regulatory proteins (ii), studying membrane association of LH-GEFs through lipid interactions in vitro and sub-cellular localization in vivo, and (iii) determining the atomic structure and overall architecture of LH-GEFs.