Dynamics of transcriptional complexes and interplay of the archaeal RNA polymerase with RNA processing proteins (A07)

我们计划研究古细菌和真核生物转录的延伸期。我们的目标是：1)在单分子水平上描述RNA聚合酶i转录机制从起始期到延伸期的结构（重组）组织；2)阐明转录和共转录过程是如何在古细菌中协调的，特别关注耦合转录和翻译机制的生化和结构特征以及含有lsm的RNPs的作用。

We plan to investigate the elongation phase of archaeal and eukaryotic transcription. We aim to i) delineate the structural (re-)organisation of the RNA polymerase I transcription machinery during the transition from the initiation to elongation phase at the single-molecule level and ii) to elucidate how transcription and co-transcriptional processes are orchestrated in Archaea with a special focus on the biochemical and structural characterisation of the coupling transcription and translation machinery and the role of Lsm-containing RNPs.