Regulatory interplay between autophagosomal and endosomal pathways (A04)

该项目的目标是连接内体通路和自噬网络的蛋白质。其中一个主要候选者是PLEKHM1，其在自噬小体-溶酶体融合调节中的作用将被分析。此外，我们将系统地筛选协调核心自噬和膜运输机制的Rab Gap蛋白。将使用CRISPR/CAS9技术进行功能性筛选。此外，我们将以秀丽线虫为模式生物，剖析Rab GAP TBC1D5在自噬、网状蛋白介导的内吞作用和神经内分泌信号中的功能作用。

This project targets proteins that link endosomal pathways and autophagy networks. One prime candidate is PLEKHM1 whose role in regulation of autophagosome-lysosome fusion will be analyzed. In addition, we will systematically screen for Rab GAP proteins that coordinate the core autophagy and membrane trafficking machineries. Functional screening will be performed using the CRISPR/Cas9 technology. Moreover, we will dissect the functional role of the Rab GAP TBC1D5 in autophagy, clathrin-mediated endocytosis, and neu-roendocrine signaling using C. elegans as model organism.