RKD factors as regulators of gametophyte development

高等植物的生命周期在二倍体孢子体和单倍体配子体之间交替进行。RKD转录因子代表了一类新的基因调控因子，例如触发卵细胞样转录组。我们目前的数据表明，拟南芥RKD基因家族的所有五个成员都是控制配子体发育所必需的。基于三个已建立的实验工具集-i)一系列RKD突变体，ii)收集细胞特异性标记系和iii)微观解剖转录组和甲基化分析-该项目专注于配子体发育过程中RKD基因网络的详细功能分析。

The life cycle of higher plants alternates between the diploid sporophytic and the haploid gametophytic generation. RKD transcription factors represent a novel class of gene regulators, which for instance trigger an egg cell-like transcriptome. Our current data suggest that all five members of the Arabidopsis RKD gene family are required for the control of gametophyte development. Based on three established experimental tool sets -i) a series of rkd-mutants, ii) a collection of cell-specific marker lines and iii) micro-dissected transcriptome and methylom Analysis- the project is focused on the detailed functional analysis of the RKD gene network during gametophyte development.