Investigation of the compositional, positional and functional heterogeneity of MICOS clusters in single mammalian cells (P01)

线粒体接触部位和嵴组织系统(MICOS)是线粒体内膜上的一个大型多亚单位复合体，是维持线粒体脊连接所必需的。MICOS复合体是连接线粒体内膜和外膜蛋白以及内质网(ER)的相互作用中心。MICOS簇在线粒体中显示出有序的排列。我们将研究线粒体运动以及分裂和融合动力学以及与内质网等细胞结构的相互作用在多大程度上影响MICOS的内部线粒体分布。因此，本项目旨在利用光学纳米显微镜和电子显微镜来研究MICOS簇相对于其亚线粒体定位的组成和功能的异质性。

The mitochondrial contact site and cristae organizing system (MICOS) is a large multi-subunit complex in the inner membrane of mitochondria that is required for the maintenance of crista junctions. The MICOS complex is an interaction hub that connects the mitochondrial inner membrane to outer membrane proteins and presumably also to the endoplasmic reticulum (ER). The MICOS clusters exhibit a well-ordered arrangement in the mitochondria. We will investigate to what extent mitochondrial movements as well as fission and fusion dynamics and interactions with cellular structures such as the ER influence the inner-mitochondrial distribution of MICOS. Thereby this project aims to investigate the compositional and functional heterogeneity of MICOS clusters with respect to their sub-mitochondrial localizations using optical nanoscopy and electron microscopy.