Protein partitioning in the synaptic compartment: principles and molecular architecture (P09)

在第一个资助期，我们分析了蛋白质的动态分配在突触前扣。在其他结果中，我们发现，可溶性蛋白质的流动性受到强烈的影响，与突触囊泡簇的相互作用。这导致突触的蛋白质组成与轴突其余部分的蛋白质组成之间的明显分离，因此充当了该系统的门，或组成防护结构。在下一个资助期，我们将分析突触后区室，重点是突触能树突棘。我们将测试两个假设：1）脊柱的3D结构，包括颈部，作为参与保留分子的扩散屏障，从而形成形态门; 2）PSD和/或脊柱细胞器影响可溶性蛋白质的定位，形成蛋白质门。我们将使用几种互补的方法，从实时成像到超分辨率成像和计算机建模。

In the first funding period we analysed the dynamic partitioning of proteins in the presynaptic bouton. Among other results, we found that soluble protein mobility was strongly affected by interactions with the synaptic vesicle cluster. This induced a clear separation between the protein composition of the synapse and that of the rest of the axon, and thus acted as the gate, or composition-proofing structure, of this system. In the next funding period we will analyse the postsynaptic compartment, focusing on the glutamatergic dendritic spine. We will test two hypotheses: 1) The 3D structure of the spine, including the neck, acts as a diffusion barrier that participates in retaining molecules, thus forming a morphological gate; 2) The PSD and/or spine organelles influence the localization of soluble proteins, forming a proteinaceous gate. We will use several complementary approaches for this, from live imaging to super-resolution imaging and computer modelling.