Reactivation of Tumor-suppressor MicroRNAs in Cancer Cells by Epigenome Editing

该项目的目标是开发一种可靠的系统，用于特定癌症相关microRNAs(MiRNAs)的靶向去甲基化和重新激活。通过采用CRISPR-Cas9系统和建立创新的编辑技术，我们已经能够准确地操纵肿瘤抑制基因miRNAs的表观基因组，用于功能分析和潜在的治疗应用。到目前为止，已经实现了以下目的：1.使用dCas9-TET1表观基因组编辑系统对肿瘤抑制基因miR-122进行靶向去甲基化2.使用miRNA表观基因组编辑鉴定miR-122靶标。MIR-122编辑通过SLC2A3靶向对肝癌细胞生长产生负面影响

The objective of this project was to develop a reliable system for targeted demethylation and reactivation of specific cancer-related microRNAs (miRNAs). By adapting the CRISPR-Cas9 system and establishing an innovative editing technology, we have been able to accurately manipulate the epigenome of tumor-suppressor miRNAs for functional analyses and potential therapeutic applications. Thus far, the following aims have been achieved:1. Targeted demethylation of the tumor-suppressor miR-122 using the dCas9-TET1 epigenome editing system2. Identification of miR-122 targets using miRNA epigenome editing3. miR-122 editing negatively impacts hepatic cancer cell growth through SLC2A3 targeting