Single-cell transcriptomic and epigenomic analysis of young and elder mouse gastric mucosa infected by Helicobacter pylori

今年，我们进行并分析了从3周龄和20周龄小鼠的胃粘膜获得的scATAC-seq数据。结果表明，在两个年龄组之间，胃上皮细胞，特别是干细胞中的染色质可及性存在差异，而表达谱相似。此外，成纤维细胞的染色质可及性随年龄的增长而保持一致，但表达谱有所不同。此外，我们从单个幽门腺中建立了类器官，并再次进行了Wnt戒断实验。来自3周龄小鼠胃窦组织的单个腺体的类器官表现出比来自散装腺体的类器官更明显的Wnt依赖性。相反，来自20周龄小鼠的类器官不受Wnt戒断的影响。

This year, we conducted and analyzed scATAC-seq data obtained from gastric mucosa of 3-week-old and 20-week-old mice. The results indicate that there are differences in chromatin accessibility in gastric epithelial cells, particularly in stem cells, between the two age groups, while the expression profiles are similar. Additionally, the chromatin accessibility in fibroblasts remained consistent with age, but the expression profiles differed.Moreover, we established organoids from single pyloric glands and conducted Wnt withdrawal experiment again. Organoids derived from single glands of 3-week-old mice antrum tissue exhibited a more pronunced Wnt-dependency than those derived from bulk glands. In contrast, organoids derived from 20-week-old mice were unaffected by Wnt withdrawal.