Analysis of key proteins involved in stomatal signaling using a novel nanobody technology.

气孔是由一对特化的保卫细胞形成的，它们调节CO2流入植物体内，减少植物的蒸腾失水。近几十年的研究揭示了质子泵、K+-和阴离子通道在调节气孔运动响应环境胁迫中的关键功能。然而，这些蛋白质在应激诱导表达过程中的蛋白质运输的关键问题仍然没有答案。这些问题将通过结合i）使用由共同主持人G. Chang，ii）质子泵、K+通道和阴离子通道SLAC 1的免疫学检测和iii）使用响应于环境刺激的真实的时间动力学监测免疫标记蛋白的蛋白运输。我将在美国加州大学圣地亚哥分校生物科学系的施罗德教授和美国加州大学圣地亚哥分校的G。张（医学院，加州大学圣地亚哥分校）在这个新颖和创新的项目取得成功。

Stomatal pores are formed by pairs of specialized guard cells regulating CO2 influx into plants and minimizing transpirational water loss of plants. Studies over the last decades revealed key functions of proton pumps, K+- and anion channels in regulating stomatal movement in response to environmental stresses. However, key questions into how protein trafficking of these proteins during stress-induced expression proceeds remain unanswered. These issues will be addressed by combining i) establishment of a powerful innovative antibody (nanobody) technology in plants using a novel nanobody technology developed by co-host Prof. Dr. G. Chang, ii) immunological detection of proton pumps, K+channels and the anion-channel SLAC1 and iii) monitoring protein trafficking of the immunologically labeled proteins using real time kinetics in response to environmental stimuli. I will pursue post doctoral research and receive excellent interdisciplinary training in the two laboratories of Prof. Dr. J. Schroeder (Division of Biological Sciences, UC San Diego) and Prof. Dr. G. Chang (School of Medicine, UC San Diego) to succeed in this novel and innovative project.