電気インピーダンスによる流路内の血球検査とマラリア検出への応用

该研究的重点是利用与日本千叶大学医院合作进行的感染疟疾寄生虫的灵长类动物，在武井实验室于2020年制造的芯片CD型EIS硬件的实验室进行深入分析验证，并在日本千叶大学医院进行有限的实地试验。因此，采用成本更低、维护更方便的人源化小鼠进行体外实验。小鼠感染是通过静脉注射途径将恶性疟原虫注射到血液中，因为静脉注射途径是稳定的，并确保寄生虫在小鼠宿主体内的长期存在。此外，每隔2 ~ 3天静脉注入新的寄生虫，可使寄生虫浓度达到10%。然后将制备好的小鼠血液在芯片CD型EIS硬件上进行不同条件下的实验。这项技术一旦成功，将在非常早期的阶段迅速发现疟疾寄生虫。

The study concentrated on intense analytical validation with a lab on chip CD type EIS hardware which was made in year 2020 in Takei lab by using malaria parasite-infected primates conducted in collaboration with Chiba University Hospital further limited field testing at Chiba University Hospital in Japan. For this reason, the humanized mouse, which is less costly and more conv enient to maintain, was used for in vitro test. The mouse is infected by injection the parasite P.falciparum into blood stream via an intravenous route because the intravenous route is stable and ensure the long-lasting presence of parasite within the mouse host. Moreover, the parasite can reach 10% of concentration by adding new parasite intravenously every 2 or 3 days. Then the we ll-prepared blood of mouse was tested by the new a lab on chip CD type EIS hardware under different conditions. This technology once successful will see prompt detection of malaria parasite at a very early stage.