Spatial organization and temporal dynamics of multi-replicon genomes in alpha-rhizobia (P07)

本项目旨在阐明苜蓿中华根瘤菌基因组DNA复制和分离与细胞生长和分裂的时空协调的分子原理。具体地说，我们将研究(I)根瘤生长和隔膜(RGS)蛋白在细胞分裂部位和最新细胞极的组装，(Ii)极性支架蛋白RgsE和POPZ在整合细胞伸长和染色体分离中的作用，(Iii)在极区和亚极区限制三个复制子的ORI的因素，(Iv)RepABS分配复合体的形成机制，以及(V)GcrA和CCRM介导的甲基化在调节次级复制子的复制启动中的作用。

This project aims at elucidating the molecular principles underlying spatio¬temporal coordination of genomic DNA replication and segregation with cell growth and division in Sinorhizobium meliloti. Specifically, we will study (i) assembly of Rgs (rhizobial growth and septation) proteins at cell division site and newest cell pole, (ii) the role of the polar scaffold proteins RgsE and PopZ in integrating cell elongation and chromosome segregation, (iii) factors involved in spatially confining the oris of the three replicons in the polar and subpolar areas, (iv) the mechanism of RepABS partition complex formation, and (v) the role of GcrA and CcrM-mediated methylation in regulating replication initiation of the secondary replicons.