The Effect of Active Zone Protein Dynamics on Synaptic Vesicle Release Probability

今年，我们一直致力于完成该软件的首次公开发布。这包括添加一些新功能，如Blender中的新可视化模块，代码现代化和一些优化。囊泡开发分支被合并到主要的开发主分支中，这意味着它现在成为STEPS的一部分，准备发布5.0版本。该软件经过了广泛的测试和验证，已在一份手稿中进行了总结，该手稿已上传到预印本服务器bioRxiv[1]。我们现在正在把这篇手稿最后提交给一家主要期刊。在建模方面，所有的模型模拟都完成了，手稿b[2]即将完成，它也将很快提交到预印本服务器。在本文中，我们以前所未有的分子和空间细节展示了完整的突触囊泡周期模型，并揭示了在高频和持续刺激期间突触传递是如何维持的。我们现在正准备把这些发现提交给一家主要杂志I Hepburn, J Lallouette, W Chen, AR Gallimore, SY Nagasawa, E De Schutter。混合囊泡和反应扩散模型与STEPS。bioRxiv 2023.05.08.539782;doi: https://doi.org/10.1101/2023.05.08.539782[2] AR Gallimore, I Hepburn， S. Rizzoli， E. De Schutter。完整突触囊泡循环的详细空间模型中囊泡池的动态调节。

This year we have been focusing on finalising the software for its first public release. This has included adding some new features such as a new visualisation module in Blender, code modernisation and several optimisations. The vesicle development branch was merged into the main developmental master branch, meaning that this now become part of STEPS ready for public release of version 5.0. The software was extensively tested and validated, as has been summarised in a manuscript that has been uploaded to preprint server bioRxiv [1]. We are now finalising submission of this manuscript to a major journal.On the modelling side, all model simulations are complete, the manuscript [2] is nearing completion and it too will be soon submitted to a preprint server. In the manuscript we present a model of the complete synaptic vesicle cycle in unprecedented molecular and spatial detail and reveal how synaptic transmission is maintained during periods of high frequency and sustained stimulation. We are now preparing to submit these findings to a major journal.[1] I Hepburn, J Lallouette, W Chen, AR Gallimore, SY Nagasawa, E De Schutter. Hybrid vesicle and reaction-diffusion modeling with STEPS. bioRxiv 2023.05.08.539782; doi: https://doi.org/10.1101/2023.05.08.539782[2] AR Gallimore, I Hepburn, S. Rizzoli, E. De Schutter. Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle.