Post-translational regulation in the Arabidopsis bZIP factor-controlled gene expression and protein-protein interaction network

在该项目的这一部分中，我们将研究拟南芥C组ZIP因子（主要是bZIP 9和63）作为S1组bZIPs的额外相互作用伙伴在响应环境条件（例如营养，缺氧，盐胁迫）的代谢中的功能。我们特别关注bZIP 63和bZIP 9磷酸化对它作为C/S1网络中转录因子的功能的影响。此外，该项目旨在使用ChIP-seq方法鉴定bZIP 63的植物靶基因，并通过在原生质体中使用基于BiFC的双杂交筛选和基于ER的酵母分裂泛素筛选来分离bZIP 63和bZIP 9的额外的非bZIP相互作用伴侣。

In this part of the project we will work on the function of Arabidopsis group C ZIP factors (mainly bZIP9 and 63) as additional interaction partners for group S1 bZIPs in reprogramming the metabolism in response to environmental conditions (e.g. nutrients, hypoxia, salt stress). We focus particularly on the implications of bZIP63 and bZIP9 phosphorylation with respect to its function as a transcription factor within the C/S1 network. Furthermore, the project aims at identifying in planta target genes of bZIP63 using a ChIP-seq approach and isolating additional, non-bZIP interaction partners of bZIP63 and bZIP9 by using a BiFC-based two-hybrid screen in protoplasts and an ER-based yeast split-ubiquitin screen.