Modular assembly, plasma membrane targeting and regulation of KCNQ (Kv7) K+ channels (C04)

人KCNQ(KV7)通道的亚基特异性组装是由含有两个螺旋序列的羧基末端结构域决定的。该项目旨在阐明KCNQ C-末端内的分子决定因素，这些决定因素驱动KCNQ亚单位的模块化组装成功能通道，确定通道模块向质膜运输的效率，并介导与调节蛋白的相互作用。在模块化概念指导下的分析将解决KCNQ通道在可兴奋细胞中组装和调节的复杂性，并对K+通道生物物理学和神经生理学产生影响。

Subunit-specific assembly of human KCNQ (Kv7) channels is determined by a carboxy-terminal domain, which contains two coiled-coil sequences. This project aims at the clarification of molecular determinants within the KCNQ C-termini, which drive the modular assembly of KCNQ subunits into functional channels, determine the efficiency of transport of channel modules to the plasma membrane, and mediate interactions with regulatory proteins. Analysis guided by the modular concept will resolve the complexity of KCNQ channel assembly and regulation in excitable cells, with implications on K+ channel biophysics to neurophysiology.