Molecular manipulation of Arabidopsis by type III effectors from the bacterial plant pathogens Pseudomonas syringae and Xanthomonas

植物病原性丁香假单胞菌和黄单胞菌。用效应蛋白重新编程植物细胞，这些蛋白通过III型分泌系统转移到植物细胞细胞质中。我们研究效应器的分子活性，目的是识别它们的植物靶标。具有ADP-核糖基转移酶(ADP-RT)活性的丁香松效应物的亚细胞定位研究表明，它们针对的是质膜和微管网络中的植物成分。我们将使用相互作用筛选和酶分析来确定植物靶标。黄单胞菌的TAL效应子结合植物基因的特定启动子DNA序列并诱导其表达。DNA结合是由一个新的、模块化的DNA结合域介导的，它决定了一种可预测的特异性。将对来自拟南芥致病黄单胞菌的TALS和人工TALS进行研究，以了解作用特异性的分子参数。

Plant pathogenic Pseudomonas syringae and Xanthomonas spp. reprogram plant cells with effector proteins that are translocated into the plant cell cytoplasm via a type III secretion system. We study the molecular activities of effectors and aim to identify their plant targets. Subcellular localization studies of P. syringae effectors with putative ADP-ribosyltransferase (ADP-RT) activity suggests that they target plant components at the plasma membrane and the microtubular network. We will identify plant targets using interaction screens and enzyme assays. TAL effectors from Xanthomonas bind specific promoter DNA sequences of plant genes and induce their expression. The DNA binding is mediated by a novel, modular DNA binding domain which determines a predictable specificity. TALs from Arabidopsis-pathogenic Xanthomonas and artificial TALs will be studied to understand the molecular parameters for specificity of action.