The influence of the microbiome and their metabolites towards epithelial functions and smooth muscle contractility in regards to InsP6 degradation

拟议项目的总体目标是确定与InsP6降解有关的微生物功能，并研究鸡上皮对肌醇(MI)的运输能力。对刺激MI摄取和肠道内稳态的代谢物及其生产者的鉴定，将为这些感兴趣的过程提供线索。此外，微生物区系(代谢物)和宿主之间的相互作用或串扰将通过一种新的方法进行研究，该方法使用Ussing小室作为短期培养系统。可能对上皮完整性和运输功能的影响将被描述。由于这是发展和维持足够的胃肠动力的生理基础，因此也是蛋鸡正常消化、生产和健康的基本前提，因此将测定平滑肌的收缩能力。因此，同时进行两种组织功能的体外测量(Ussing小室，等长力测量)将使人们能够第一次洞察肠道微生物区系和宿主组织之间相互作用的潜在途径。该项目与研究股的其他项目有密切联系。本研究中使用的动物不属于项目1中描述的主要动物实验，因为整个项目期间都需要新鲜的组织材料。然而，动物的品种、饮食和生命周期足以满足主要实验的需要。项目4中广泛使用了表征微生物区系的方法。MI转运研究将与项目5中提议的工作密切相关。Ussing小室实验中确定的肠道屏障功能可以与项目7中获得的结果相结合。

The overall aim of the proposed project is to identify microbial functions in regards to InsP6 degradation and to study the capacities of the chicken epithelium for myo-inositol (MI) transport. The identification of metabolites and their producers, which stimulate the MI uptake and the gut homeostasis, will throw light on these processes of interest. In addition, the interaction or cross-talk between the microbiota (metabolites) and the host will be investigated by a novel approach using Ussing chambers as short-term incubation systems. Possible effects towards epithelial integrity and transport functions will be described. Smooth muscle contractility will be determined as this is the physiological basis to develop and maintain adequate gastrointestinal motility and is thereby, an essential prerequisite for proper digestion, performance and health of laying hens. Thus, simultaneous performing of two in vitro measures of tissue functions (Ussing chambers, isometric force measures) will allow to reveal a first insight in potential pathways of the cross-talk between gut microbiota and host tissues.The project has close connections to other projects of the Research Unit. Animals used in this study are outside the main animal experiments described in project 1 as fresh tissue material is needed throughout the project period. However, animal breeds, diets and periods of life are adequate to the main experiments. Methods to characterize the microbiota are extensively used in project 4. MI transport studies will be carried out in close association to work proposed in project 5. Intestinal barrier functions determined in Ussing chamber experiments can be combined with results obtained in project 7.