Electron Microscopy

电子显微镜(EM)是唯一可以成像TM系统结构的技术，根本没有其他方法来可视化这个膜系统的复杂性，并在纳米尺度上定位其中的蛋白质。关于突变体或不同发育阶段的TM超微结构和蛋白质定位的问题是研究单位所有小组的核心问题。我们将为所有小组提供熟练的电子显微镜技术支持，帮助他们回答关于TM超微结构和其中蛋白质的空间组织的问题。在第一个资助期，我们确实建立了一个高吞吐量的工作流程，使用化学固定和塑料嵌入的样品来分析TM拓扑结构。使用优化方案的这种超微结构分析也将在第二个资助期发挥重要作用，并将通过免疫金技术对蛋白质进行定位的工作流程加以扩展。

Electron microscopy (EM) is the only technique which allows to image the arch¬itecture of the TM system, there is simply no other way to visualize the complexity of this membrane system and also localize proteins therein at the nanoscale. Questions regarding TM ultrastructure in mutants or different developmental stages and the localization of proteins are central for all groups of the Research Unit. We will provide skilled technical support for transmission electron microscopy for all groups to help them answer their questions regarding TM ultrastructure and spatial organization of proteins therein. During the first funding period we did establish a high through-put workflow for the analysis of TM topology using chemically fixed and plastic embedded samples. Such ultrastructural analyses using optimized protocols will play an important role also during the second funding period and will be extended by a workflow for the localization of proteins by immunogold techniques.