The development of multi-functional enzyme-based therapy against Triple Negative Breast Cancer

首先，我想鉴定针对巨噬细胞上表达的集落刺激因子-1受体的大环肽。肿瘤细胞释放的巨噬细胞集落刺激因子激活免疫刺激性巨噬细胞，使其极化为免疫抑制性肿瘤相关巨噬细胞。应用RaPID mRNA展示来发现固定在链霉亲和素磁珠上的生物素化MCSF的肽结合物。进行连续五轮的选择，包括用MCSF固定化珠的阳性轮和用空生物素掩蔽的链霉亲和素磁珠的阴性轮。基于下一代测序结果，大多数获得的结合物结果未显示阳性和阴性轮次之间的差异。

Firstly, I wanted to identify the macrocyclic peptides against Colony-Stimulating Factor-1 Receptor expressed on the macrophage. The activation from macrophage colony stimulating factor emitted form cancer cell would induce the polarization of immunostimulatory macrophage to immunosuppressive Tumor-Associated Macrophage. The RaPID mRNA display was applied to discover the peptides binder to biotinylated MCSF immobilized on streptavidin magnetic beads. The selection was conducted for five consecutive rounds including the positive rounds with MCSF immobilized beads and negative rounds with empty biotin masked streptavidin magnetic beads. Based on the next generation sequencing results, the majority of acquired binders results did not show difference between positive and negative rounds.