Functional expression of mammalian ion channels and analysis of the molecular mechanisms

离子通道蛋白响应于各种刺激而用于离子的选择性渗透并调节活生物体中的电信号。为了理解这些通道的分子机制，需要具有原子分辨率的精确结构信息。 在这项研究中，哺乳动物的离子通道蛋白，这是在医学和制药科学的重要目标，已研究了衰减全反射（ATR）傅立叶变换红外光谱，为我们提供精确的分子信息。 在筛选了几种离子通道蛋白后，我们利用ATR FT-IR光谱成功解析了参与细胞静息膜电位调节的KCNK 1通道选择性过滤器的酰胺I谱带。

Ion channel proteins work for selective permeation of ions in response to various stimuli and regulate electrical signals in living organisms. To understand the molecular mechanisms of these channels, the precise structural information with atomic resolution is required. In this study, mammalian ion channel proteins, which are important targets in medical and pharmaceutical sciences, have been studied by attenuated total reflection (ATR) FT-IR spectroscopy which gives us precise molecular information. After screening of several ion channel proteins, we have succeeded to resolve amide I bands of selectivity filter of KCNK1 channel, which is involved in regulation of resting membrane potential of cell, by using ATR FT-IR spectroscopy.