Developmental transcriptome analysis of monkey brain for the establishment of human disease model

为了建立人类神经精神疾病和高级认知功能的灵长类动物模型，我们对猕猴进行了涵盖12个脑区和7个发育阶段的时空脑转录组分析。下一代测序仪的高通量转录组分析表明，个体内脑区之间的基因表达谱比同一脑区之间的基因表达谱更相似。我们还旨在阐明单个神经元水平的区域和电路特异性的分子基础。为了实现这一目标，将三种不同的荧光逆行示踪剂注射到猴子大脑的三个不同部位（丘脑、纹状体、脑桥），并在前额叶区域成功地确认了神经元上的三种不同信号。目前，我们将利用激光捕获显微解剖的方法，在荧光信号的基础上，对每个神经元进行采集，以描绘区域和电路特异性神经元的分子特征。

For the establishment of primate model of human neuropsychiatric diseases and high-level cognitive functions, we performed spatio-temporal brain transcriptome analysis of macaques covering 12 brain regions and 7 developmental stages. High throughput transcriptome analysis by next generation sequencer reveals that profile of gene expression is more similar between brain regions within individuals than between individuals in the same brain regions.We also aimed to elucidate the molecular basis of regional and circuit specificities at the single neuron level. To execute this, three different fluorescent retrograde tracers were injected into three distinct part of monkey brains (thalamus, striatum, pons), and three distinct signals on neurons were successfully confirmed in the prefrontal regions. At this moment, we are going to profile molecular signatures of regional and circuit specific neurons by collecting each neuron based on the fluorescent signal by laser capture microdissection.