Electron micrograph imaging of a single protein without staining

本研究的目的是利用扫描电子显微镜(STEM)对单个蛋白质分子进行不染色成像。本研究的关键技术是使用氧化石墨烯(GO)薄膜作为STEM观察的衬底。目标分辨率为1 nm，在溶菌酶蛋白水平。该项目的成果是：1)在铜微网上开发了单层GO膜。2)在低加速电压下制备GO单层蛋白质样品而不损失分辨率。3)在GO膜上未见铀酰染色的单一NADH泛醌还原酶的STEM监测，其大小为20 nm。

The object of this research is an imaging of a single protein molecule by a scanning transmission electron microscope (STEM) without staining. The key technology of this research is using a graphene oxide (GO) film as a substrate for STEM observation. The target resolution is 1 nm in the level of lysozyme protein. The outcome of the project were1) Development of a single layer GO film on a Cu microgrid. 2) Preparation of protein sample on a GO single layer under low acceleration voltage without loss of resolution. 3) STEM monitoring of a single NADH ubiquinone reductase whose size is 20 nm without uranyl staining on a GO film.