Chemical Biology on the Symbiotic System in Marine Invertebrates

已有研究表明，海洋次生代谢物大多来源于共生微生物。在本研究项目中，我们从海洋海绵花瓣中分离出一种独特的海洋代谢物--花蕾盘菌素A，并对其产生菌进行了分离和鉴定。我们已经成功地获得了含有被认为产生毛盏花素A的浓缩微生物的组份，并从这组浓缩微生物中提取了基因组DNA。提取的基因组DNA的后基因组DNA测序分析和后续的DNA定位使我们能够鉴定出PKS基因簇的主要微生物成分和13个重叠群。

It has been disclosed that most of the marine secondary metabolites originate from the symbiotic microbes. In this research project, we focused on an unique marine metabolite, calyculin A from a marine sponge Discodermia calyx, and tried to separate and identify the calyculin A producing microbe. We have succeeded to obtain the fraction with concentrated microbes thought to produce calyculin A and extraction of genomic DNA from this assembly of the concentrated microbes. The metagenomic DNA sequencing analysis of the extracted genomic DNA and the following DNA mapping allowed us to identify the major microbe constituents and 13 contigs of PKS gene clusters.