Designing and imaging of reconstructed muscle using DNA nanostructure

我们的目标是通过创造一个由蛋白质和DNA分子组成的人造肌肉，并在单分子分辨率下观察其动力学，来阐明肌肉的节能、灵活性和适应性的机制。我使用了名为DNA折纸的DNA纳米技术，将纳米尺寸的模块与马达蛋白分子杂交。我成功地构建了亚微米尺寸的人造运动蛋白组件。通过这次实验，我确定了控制马达分子数量和间距的方法学。这种新颖的方法论使我们能够分析生命独特属性的产生机制。

Our goal is to clarify the mechanism of energy-saving, flexibility and adaptability of muscle by creating an artificial muscle made of proteins and DNA molecules and observing the dynamics at the single molecule resolution.I used DNA nanotechnology called DNAorigami and the nanosized modules were hybridized with motor protein molecules. I succeeded in constructing the artificial motor protein assembly with sub-micrometer size. Through this experiment, I confirmed the methodology for controlling the number and spacing of motor molecules. This novel methodologies enables us to analyze the emergence mechanism of unique properties of life.