19F-MR Imaging of Nucleic Acids based on Photochemically Hyperpolarlized Nuclear Spins

体内成像技术，如X射线计算机断层扫描、正电子发射断层扫描和核磁共振成像，是诊断各种疾病和手术治疗后随访的有前途的技术。由于19F-MR在体内的低本底信号，这些基于19F-MR的成像探针具有高对比度和靶向生物分子的特异性成像的潜力。然而，由于~(19)F磁共振信号的灵敏度较低，能够对核酸进行成像的分子~(19)F探针还鲜有报道。作为一种利用19F原子核的对氢诱导超极化(PhIP)极大地增强19F原子核磁共振信号的方法。因此，本文研究了基于DNA交联的环丁烷二聚体光化广义对氢的DNA~(19)F-MR成像。

In vivo imaging technologies, such as X-ray computed tomography, positron emission tomography, and nuclear magnetic resonance imaging, are promising technologies for diagnosis of various disorders and follow up after surgical treatments. Due to the low background signal of 19 F MR in vivo, these 19F MR based imaging probes have potential for high contrast and specific imaging of target biomolecules. However, molecular 19F probes that can image nucleic acids have been scarcely reported, because of low sensitivity of 19F MR signal. As the method that can tremendously enhance the MR signal of 19F nuclei by using of parahydrogen-induced hyperpolarization (PHIP) of 19F nuclei. So, 19F-MR imaging of DNA based on photochemically generalized parahydrogen caused from cyclobutane dimer by DNA crosslinking was investigated.