Mapping of mutation point on holokinetic chromosome in Bombyx mori

我对国家生物资源计划（NBRP）提供的3株家蚕（r501、r52和r53）染色体突变进行了分析。BAC-FISH（利用细菌人工染色体的荧光原位杂交）揭示了23号和25号染色体之间融合的确切方向。我定义了反向易位是r52和r53菌株突变的原因。用BAC-FISH分析了每条突变染色体的双链断裂和进一步融合情况。这些检测到导致染色体突变的桥式BAC。我试图通过PCR揭示突变点，作为家蚕基因组数据的参考。

I have analyzed chromosome mutation in 3 Bombyx mori strains (r501, r52 and r53) provided through national bioresource project (NBRP). BAC-FISH (fluorescence in situ hybridization using bacterial artificial chromosomes) revealed the exact direction of the fusion between chromosomes 23 and 25. I defined reciprocal translocation is the cause for the mutation in r52 and r53 strains. The double strand breakage and further fusion for each mutation chromosomes were also analyzed by the BAC-FISH. These detected a bridge BAC responsible for a mutation chromosome. I am trying to reveal the mutation point by PCR as a reference of Bombyx mori genome databes.