Identification of target proteins for ubiquitination in brown adipose tissue of fasted rat

为了阐明特定蛋白质降解调节能量代谢的机制，我们试图鉴定大鼠棕色脂肪组织中响应能量水平的泛素化蛋白。将7周龄雄性Wistar大鼠限食1周，然后分为2组，分别为进食组和禁食组。给大鼠注射环氧霉素，一种有效的蛋白酶体抑制剂。确定环氧霉素的给药剂量和给药方法，以检测两组之间泛素化蛋白水平的差异。

To clarify the mechanism for energy metabolism regulation by specific protein degradation, we attempt to identify the proteins that ubiquitinated in response to energy level in rat brown adipose tissue. Male Wistar rats aged 7 weeks old were subjected to 1 week restricted feeding, and then divided into 2 groups, fed and fasted. The rats were administrated epoxomicin, a potent proteasome inhibitor. The dose and method for administration of epoxomicin were determined to detect the difference of ubiquitinated protein levels between the 2 groups.