Investigation of target proteins by isothiocyanate with fluorescent probe

虽然异硫氰酸酯具有一定的生物学功能，如抗肿瘤、抗菌等，但其作用机制还不清楚。我们发现在生理条件下ITC与蛋白质中的赖氨酸共价加合。本研究利用荧光标记技术对ITC的荧光特性进行了研究。制备了一种抗荧光结构的单克隆抗体，并对其进行了表征，应用于细胞的荧光染色。然后通过荧光扫描或通过特异性抗体在凝胶中检测多个ITC加合蛋白。ITC加合蛋白的鉴定仍有待完成。

Though isothiocyanates have some biological functions such as anti-cancer or anti-bacterial activities, the mechanisms has not been shown in detail. We found the covalent adduction of ITC with lysine in a protein at physiological conditions. In this study, we focused in fluorescent character of ITC by using FITC. A monoclonal antibody to fluorescent structure has been prepared and characterized and then applied to fluorescent staining of cells. The multiple ITC adducted proteins were then detected in a gel by fluorescent scanning or by specific antibody. The identification of ITC adducted proteins remains to be done.