Utilization of entomophilic nematodes as carriers of entomopathogenic microbes

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The nematode fauna associated with the Japanese pine sawyer, Monochamus alernatus was examined. In total, six species on nematodes including pine wood nematode, Bursaphelenhcus xylophilus and Contortylenchus genitalicala were isolated. Within these nematode species, undescribed Diplogasteroides sp. was considered as a candidate for the carrier of entomopathogenic microbes. The nematode species was then, described as D. andrassyi n. sp., and feeding experiment was conducted, i.e., the nematode's ability to carry the microbes were examined. The experiment suggested that the nematode species which has wider stoma can be more effective carrier than D. andrassyi.