The research on flavivirus organ-tropism

为了巩固病原微生物的器官嗜性，我们使用嗜神经黄病毒作为感染因子的模型进行了如下所述的实验。首先，制备在其基因组RNA内编码红色荧光蛋白基因的黄病毒报告病毒颗粒（RVP），使其成为可见的病毒感染。其次，建立了小鼠脑组织切片培养体系，便于分析病毒的器官嗜性。建立了黄病毒RVPs感染小鼠脑片的实验体系。该系统可用于病原微生物嗜性的研究。

In order to solidify organ-tropisms of pathogenic microbes, we conducted the experiments as described below, using a neurotropic flavivirus as a model of infectious agent. First, flavivirus reporter virus particles (RVPs) encoding the red-fluorescent protein gene within its genome RNA, were prepared to be visible virus infections. Next, mouse brain slice-culture system was established to analyze organ-tropism of the virus easily. Finally, flavivirus RVPs-infection system on mouse brain slice-cultures was established. We might apply the system to do research on organ-tropism of pathogenic microbes.