Biological roles of fatty acid composition of phospholipids.

在这项研究中，我们研究了脂肪酸重建的机制及其生物学作用。我们重点关注两种磷脂酶，即iPLA 1a和iPLA 1g。我们在HeLa细胞中通过RNAi技术敲低了两个iPLA 1，并检测了细胞的脂肪酸组成。我们发现，在sn-1位置的磷脂的脂肪酸重塑显着延迟iPLA 1a敲除HeLa细胞。此外，在iPLA 1a敲除细胞中经常观察到异常线粒体。这些结果表明，在磷脂的sn-1位的脂肪酸重构的iPLA 1a在线粒体的功能中具有关键作用。

In this study, we examined the mechanism of fatty acid remodeling and its biological roles. We focused two phospholipases called iPLA1a and iPLA1g. We knockdowned the two iPLA1s by RNAi technique in HeLa cells and examined the fatty acid composition of the cells. We found that fatty acid remodening of phospholipid at the sn-1 position was dramatically delayed in iPLA1a-knockdowned HeLa cells. In addition, abnormal mitochondria were frequently observed in the iPLA1a-knockdowned cells. These results indicate that fatty acid remodeling at the sn-1 position of phospholipids by iPLA1a has a critical role in the function of mitochondria.