Development of drugs targeting hepatitis B x-protein

我们开展了一项研究项目，开发新型乙型肝炎病毒（HBV）抗病毒药物。在研究期间，我们获得了以下结果：(1)选择性缺失HBX蛋白的HBV表达质粒转染人肝癌Huh7细胞后表达水平较野生型降低。(2)构建HBV突变表达质粒，在HBX蛋白编码框中连续引入4次丙氨酸替代。利用突变体HBX质粒，我们在HBX的c端发现了AP1反激活子结构域。

We have conducted study project to develop new classes of antiviral drug against hepatitis B virus (HBV). In the study period, we obtained the following results. (1) HBV expression plasmid which selectively lacks HBX protein showed decreased expression levels as compared with wild type when transfected into humans hepatoma Huh7 cells. (2) Mutant HBV expression plasmids were constructed in which 4 consecutive alanine substitution were introduced in the HBX protein coding frame. By using the mutant HBX plasmids, we found AP1 transactivator domain in C-terminal of HBX.