Identification of upstream gene of psoriasis and the architecture of psoriatic epidermis by crosstalk between epidermal keratinocytes and blood cells in vitro

寻常型银屑病是一种慢性炎症性皮肤病。其发病机制尚不完全清楚。SLULP-2是一种分泌型蛋白，是一种分泌型蛋白，在银屑病皮损中显著上调。构建SLULP-2/FLAG表达载体，并将其导入正常人表皮角质形成细胞。转染的角质形成细胞显示银屑病中上调的促炎细胞因子或抗菌肽的过度表达。这些细胞与健康受试者的血细胞共同培养。在三维培养体系中，用共培养上清液培养的表皮角化细胞形成棘皮构筑。这些发现提示SLULP-2可能参与了银屑病的病理生理过程。

Psoriasis vulgaris is one of chronic inflammatory skin diseases. Its pathogenesis remains to be completely elucidated. SLURP-2, a secreted Ly-6/uPAR related protein-2 is a secretory protein, which is significantly upregulated in the psoriatic lesion. SLURP-2/FLAG expression vector was constructed, followed by the transfection into normal human epidermal keratinocytes. The transfected keratinocytes showed the overexpression of the proinflammatory cytokines or antimicrobial peptides that are upregulated in psoriasis. Those cells were co-cultured with blood cells from healthy subjects. The epidermal keratinoyctes in three-dimensional culture system, which were cultivated with the supernatant of co-culture, resulted in acanthotic epidermal architecture. These findings suggest that SLURP-2 may be involved in the pathophysiology of psoriasis.