Development of novel methods for imaging of bone marrow utilizing fluorescence techniques and spatiotemporal analysis of bone metastasis

为了阐明肿瘤细胞与微环境相互作用的分子机制，确定肿瘤骨转移的溶骨/成骨倾向，通过一系列实验建立了一套体内成像系统，可在多个时间点同时分析骨髓中的4种细胞、癌细胞、破骨细胞、成骨细胞和骨细胞。将表达荧光蛋白的癌细胞移植到小鼠体内，建立了溶骨性前列腺癌的骨转移系统。对破骨细胞进行体内荧光成像。

To elucidate the molecular mechanisms of interaction between cancer cells and microenvironments which determine the osteolytic/ osteogenic tendencies of bone metastases of cancer, series of experiments were performed to establish an in vivo imaging system by which 4 kind of cells, cancer cells, osteoclasts, osteoblasts and osteocytes in bone marrow can be analyzed simultaneously at multiple time points. Cancer cells expressing fluorescent proteins were implanted to mice and bone metastasis system of osteolytic prostate cancer was established. In vivo fluorescence imaging for osteoclast was also performed.