Induction of Mandibular bone and tooth germ in embryo body formation from mouse and human iPS cells in serum-free cell culture

miPS细胞已在ESF 7无血清培养基中维持超过2年，具有未分化的表型。FGF-2诱导miPS细胞分化为牙胚祖细胞。hiPSC通常维持在补充有FBS的培养基中的饲养细胞上。在这项研究中，我们成功地产生hiPSCs从人牙髓细胞使用Oct 3/4，Sox 2，Klf 4，c-Myc与逆转录病毒载体在血清和饲养层定义的培养条件。这些hiPSC保留了自我更新的性质，并且通过胚胎体形成测定和畸胎瘤形成测定通过分化成所有三个初级胚层的衍生物来评价多能性。在hESF 9培养基中培养的hiPSC绝对需要TGF-β 1来维持多能性。这种简单的无血清贴壁单培养系统将使我们能够阐明细胞在特定条件下对生长因子的反应，并可以消除未知病原体可能带来的风险。

The miPS cells have been maintained in ESF7 serum-free medium for more than 2 years with an undifferentiated phenotype. An FGF-2 induced miPS cell differentiation into tooth germ progenitor cells. hiPSCs are commonly maintained on feeder cells in medium supplemented with FBS. In this study, we successfully generated hiPSCs from human dental pulp cells using Oct3/4, Sox2, Klf4, c-Myc with retroviral vectors in serum- and feeder-free defined culture conditions. These hiPSC retained the property of selfrenewal, and pluripotency evaluated by differentiation into derivatives of all three primary germ layers by embryo body formation assay and teratoma formation assay. hiPSCs cultured in hESF9 medium absolutely required TGF-beta1 to maintain pluripotency. This simple serum-free adherent monoculture system will allow us to elucidate the cell responses to growth factors under defined conditions and can eliminate the risk might be brought by undefined pathogens.