A pilot study for visualizing diurnal hormonal secretion pattern within a hair

激素显示出特征性的昼夜分泌模式，早上较高，晚上较低。血液中的这种昼夜激素分泌可能会记录在头发中，就像“树木的年轮”一样，因为头发的生长，每天约0.3毫米，涉及体内最活跃的细胞活动之一。这项研究的挑战是通过使用生物化学分析，如蛋白质染色和免疫染色来可视化这种昼夜模式。这一目标迄今尚未实现。然而，积累了技术和知识以达到最终目标，例如开发了毛发切片制作技术和毛发切片的蛋白质染色程序，并观察了详细的激素昼夜模式，特别是在夜间到早晨。

The hormones shows a characteristic diurnal secretion pattern, the higher in the morning and lower in the evening. This diurnal hormonal secretion in the blood might be recorded within a hair just like as "an annual ring of tree," because the hair growth, which is about 0.3 mm every day, is involved one of the most active cellular activity in the body. The challenge of this study is to visualize this diurnal pattern by using biochemical analysis, such as protein staining and immunostaining. This objective has yet to be achieved so far. However, there accumulated the techniques and knowledge to reach a final goal, such as development of making hair section technique and protein staining procedure for the hair section, and observation of detailed hormonal diurnal patter, especially during night through morning.