Specificity of the GET pathway for TA protein insertion in A. thaliana (A06*)

该项目将包括AtGET 3a-TA蛋白结合位点的详细结构/功能分析。它不仅有助于识别特异性结合基序，而且这些基序也将有助于理解和阐明AtGET 3a的替代功能。这是特别感兴趣的，因为在酵母中执行ATP非依赖性伴侣作用的一些重要基序在古质体GET 3a中不保守。此外，异位表达AtGET 3a-GFP在受体功能丧失Atget 1中的严重表型将允许解决AtGET 3a在膜蛋白插入或一般伴侣活性中的替代功能之间的机械差异。

This project will comprise a detailed structure/function analysis of AtGET3a-TA protein binding sites. It will not only help to identify specific binding motifs, but these motifs will also help to understand and elucidate alternative functions of AtGET3a. This is of particular interest as some important motifs that execute ATP independent chaperone action in yeast are not conserved in Archaeplastida GET3a. In addition, the severe phenotype of ectopically expressed AtGET3a-GFP in the receptor loss-of-function Atget1 will allow resolving mechanistic differences between the alternative functions of AtGET3a in either membrane protein insertion or general chaperone activity.