Mechanics of Topological Rearrangements during DNA Replication (A12*)

细胞DNA复制机制如何克服染色体上遇到的障碍，同时避免损伤和基因组不稳定性仍然知之甚少。在这里，我们将使用单分子成像方法来研究这些事件的动态，以直接观察在遇到拓扑障碍时的复制动力学。这些结果将阐明拓扑应变对复制体动力学的影响，以及DNA复制机制和环状ATP酶（SMC）之间碰撞的后果，这些ATP酶在染色体组织和分离中起着至关重要的作用。

How the cellular DNA replication machinery overcomes obstacles encountered on chromosomes while avoiding damage and genome instability remains poorly understood. Here, we will study the dynamics of these events using single-molecule imaging approaches to directly observe the kinetics of replication during encounters with topological barriers. The results will clarify the influence of topological strain on replisome dynamics and the consequence of collisions between the DNA replication machinery and ring-shaped ATPases (SMCs) that play vital roles in chromosome organisation and segregation.