Visualization of in vivo RNA behavior by direct labeling of RNA

DNA的遗传信息被转录成信使RNA，然后翻译成蛋白质。在许多情况下，信使RNA不是悬浮在细胞质中，而是定位在细胞骨架上，并运输到邻近的细胞。虽然定位和移动对基因表达很重要，但还没有建立起在活细胞中观察RNA定位的好方法。在本研究中，我们使用激光辅助显微注射器，并尝试注射荧光标记的番茄花叶病毒RNA，以建立活细胞中的RNA可视化。遗憾的是，我们还没有在显微注射荧光标记的RNA方面取得成功的结果

Genetic information of DNA is transcribed to messenger RNAs, and then translated to proteins. In many cases, messenger RNAs are not suspended in cytoplasm, but localize on cytoskeletons and transported to neighboring cells. Although the localization and movement is important for gene expression, no good method for seeing RNA localization in living cells has been established. In this study, we used laser-assisted microinjector and tried to inject fluorescent-labeled tomato mosaic virus RNA for establishment of RNA visualization in living cells. Unfortunately we have not yet obtained successful results on microinjection of the fluorescent-labeled RNA