Analysis of function of endosymbiotic bacteria, Wolbachia, genes on the genome of Monochamus alternatus.

松墨天牛是一种天牛，作为松材线虫的媒介而臭名昭著，这种线虫会引起松材线虫病。这种昆虫在常染色体上腐蚀许多沃尔巴克氏菌基因。在本研究中，从昆虫的每个组织中提取RNA，进行转录组分析，以明确沃尔巴克氏菌基因的表达情况。在每个组织中检测到了几个疑似沃尔巴克氏菌基因的阵列，阵列的数量在不同组织之间存在显著差异。这一结果表明，转移到松墨天牛体内的一些沃尔巴克氏菌基因已经在该昆虫中表达。

Monochamus alternatus is the longicorn beetle notorious as a vector of the pinewood nematode that causes the pine wilt disease. This insect caries many Wolbachia genes on an autosome. In this study, The RNA was extracted from each tissue of the insect, transcriptome analysis was conducted to clarify the presence or absence of the expression of Wolbachia genes. Several arrays that seem to be derived from Wolbachia genes in each tissue were detected, the number of the arrays was different between the tissues remarkably. This result suggests that some of the Wolbachia genes transfered to M.alternatus have expressed in the insect.