Study on regulatory mechanisms of coloration of fruit of fruit trees by blue light

为了研究蓝光照射对果实发育期后果实颜色发育的影响，本文以“Kyoho”（Vitis vinifera L. x Vitis labrusca L.）植株为材料，分析了花青素的积累及其机制。用蓝光LED照射葡萄果实，研究了蓝光LED对葡萄果皮颜色的影响。结果表明，蓝光通过生物合成酶活性促进花青素合成，尤其是类黄酮3’5’羟基化酶（F3’5’h）。在离体葡萄和土栽葡萄树上进行的辐照实验表明，蓝光照射可诱导花青素积累。F3'5'H基因的上游区域包含几个顺式转录因子的基序序列。这些结果表明，蓝光照射将是一种改善葡萄皮颜色的方法。

To investigate the effects of blue light illumination on fruit color development after verason of fruit development, anthocyanin accumulation and mechanisms of it were analyzed using 'Kyoho' (Vitis vinifera L. x Vitis labrusca L.) plants. Grape berries were illuminated by blue LED and the effect on peel color of grapes was investigated. The result indicated that blue light promotes anthocyanin synthesis through biosynthetic enzyme activities, especially, flavonoid 3'5' hydroxilase (F3'5'H). An irradiation experiments using in vitro grape and soil planted grape trees revealed that anthocyanin accumulation was induced by blue light irradiation. The upstream region of the F3'5'H gene contains several motif sequences for cis-transcriptional factors. These results suggest that blue light irradiation would be a way to improve coloration of grape peel.