Dynamics of nuclear architecture involving in reprogramming of mouse somatic nuclear transfer embryos

研究了体细胞核移植(SCNT)胚胎早期发育过程中染色质组成的动态变化。结果如下：(1)SCNT胚胎的组蛋白组成与受精胚胎不同；(2)组蛋白脱乙酰酶(HDAC)抑制剂可将组蛋白变体从染色质中去除；(3)组蛋白修饰和处置的反应性取决于HDAC抑制剂和/或细胞类型。

This study examined the dynamics of chromatin composition in the early development of somatic cell nuclear transfer (SCNT) embryos. The results are summarized as follows: (1) histone composition of the SCNT embryos differs from that of the fertilized ones, (2) a certain histone variant is removed from chromatin by histone deacetylase (HDAC) inhibitors, and (3) responsiveness of histone modification and disposition depends on HDAC inhibitors and/or the types of cells.