Development of genetically engineered animals by use of non-B DNA structure for highly and stably expression of transgene

本研究旨在探讨非B DNA序列对RNAPII和/或RNAPIII转录激活的作用，以开发高效稳定表达转基因的转基因动物。结果表明：T20（180 bp人工弯曲DNA）作为非BDNA序列的模型，显示了RNAP Ⅱ对小鼠ES细胞转录的激活作用，显示了ES细胞体外分化过程中转录和染色质动力学的稳定性，以及T20驱动转基因小鼠产生的可能性。

This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows : T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.