Development of genetically engineered animals by use of non-B DNA structure for highly and stably expression of transgene

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This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows : T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.