Small non-coding RNAs in Streptomyces coelicolor

天蓝色链霉菌是一种革兰氏阳性、富含GC的土壤细菌，是研究次生代谢物产生(抗生素)和细胞分化的模式生物。我们的研究主题是分析这种生物体中的小调控RNA(SRNA)。在上一次筹资期间，我们描述了sna sc1的特点。它通过与靶mRNA的核糖体结合部位直接相互作用来控制琼脂糖酶DAGA的表达。此外，sc1还参与氮代谢并影响抗生素的产生。我们的目标是进一步确定SC1的靶点，并揭示其在次生代谢控制中的作用。我们使用深度测序的方法进一步确定了大约800个潜在的sRNA，并选择了一组8个sRNA进行进一步分析。它们在最低限度的介质中有不同的表达，分别表现出氮或温度依赖性。我们将对这些候选者进行详细的结构和功能描述。进一步，我们试图确定参与sRNA-mRNA相互作用的蛋白质，因为天蓝色链霉菌缺乏主要的伴侣Hfq，而Hfq在其他生物中促进这种相互作用。

Streptomyces coelicolor is a Gram-positive, GC-rich soil bacterium that serves as a model organism for the investigation of secondary metabolite production (antibiotics) and cell differentiation. Our research topic is the analysis of small regulatory RNAs (sRNAs) in this organism. During the last funding period we characterized the sRNA sc1. It controls the expression of the agarase DagA by a direct interaction with the ribosomal binding site of the target mRNA. Moreover, sc1 is involved in nitrogen metabolism and influences the production of antibiotics. We aim to characterize further targets of sc1 and unravel its role in the control of secondary metabolism. We have identified further ~ 800 potential sRNAs using a deep sequencing approach and selected a set of eight sRNAs for further analysis. They are differentially expressed in minimal media and show nitrogen or temperature dependence, respectively. We will perform a detailed structural and functional characterization of these candidates. Further on, we attempt to identify proteins involved in the sRNA-mRNA interactions since S. coelicolor lacks the major chaperon Hfq which facilitates this interaction in other organisms.