Diagnoses of animal diseases by monoclonal antibodies

1. 肉毒中毒：对纯化的肉毒梭菌轻、重链毒素<C_1>和D产生单克隆抗体。利用单克隆抗体将日本保存的所有肉毒杆菌毒素分为4组。小鼠肝炎（MH）：制备了针对MH病毒分离株的单克隆抗体。一些单克隆抗体与病毒聚合体特异性反应，其余单克隆抗体与病毒核酸特异性反应。所有获得的单克隆抗体均与所有MH病毒株发生交叉反应。马立克氏病（MD）：对从MD肿瘤中建立的淋巴样细胞系的表面抗原制备单克隆抗体。单克隆抗体与MD鸡的肿瘤细胞和表面健康的鸡的外周血细胞有相同的反应。反应阳性鸡的肿瘤细胞和血细胞中阳性细胞的比例相对较低。猪瘟（HC）：用白血病猪和HC免疫猪的肿瘤细胞组成的系统和其他一些系统建立杂交瘤失败。然而，最近用感染丙型肝炎的猪的肾细胞免疫小鼠的脾细胞获得了单克隆抗体阳性细胞。流感和副粘病毒感染：利用单克隆抗体对流感病毒的HA或NA进行检测，可以清楚地证明流感病毒的抗原改变和重组的起源。利用抗副粘病毒单克隆抗体，证实了副粘病毒抗原表粒的稳定性和抗原改变。牛白血病：建立抗牛地方性白血病肿瘤相关抗原（EBL）的单克隆抗体，与临床白血病奶牛的所有肿瘤细胞特异性反应。这一发现提示了利用单抗诊断ebl的可能性。感染性胰腺坏死：产生部分产生单克隆抗体的细胞。然而，细胞的维护失败了；由于细胞不能传代或细菌污染。

1. Botulism: Monoclonal antibody (MAb) was produced against purified toxins <C_1> and D, their light and heavy chains of Clostridium botulinum. By the use of MAbs, toxins of all the C. botulinum, which were stored in Japan, were divided into 4 groups.2. Mouse hepatitis (MH): MAbs were prepared against an isolate of MH virus. Some of the MAbs specifically reacted with virus peplomer, and the remaining ones with virus nucleic acid. All the MAbs obtained cross-reacted with all the MH virus strains tested.3. Marek's disease (MD): MAbs were produced against surface antigens of a lymphoid cell line established from MD tumor. The MAbs reacted equally with tumor cell of chickens with MD and with peripheral blood cells of apparently healthy chickens. In reaction-positive chickens, ratio of positive-cell was relatively low both in the tumor and blood cells.4. Hog cholera (HC): Establishment of hybridoma was unsuccessful by the system consisted of tumor cell from pigs with leukosis and HC-immunized pigs, and by some other systems. However, MAb-positive cells have recently been available using spleen cell of mice immunized with kidney cells from HC-infected pig.5. Influenza and paramyxovirus infection: Antigenic alteration and the origion of a recombinant of influenza virus were clearly demonstrated by using MAbs against HA or NA of the virus. By the use of anti-paramyxovirus MAb, the stability of epitom and antigenic alteration of paramyxovirus were proved.6. Bovine leukosis: MAb established against tumor-associated antigen of enzootic bovine leukosis (EBL) specifically reacted with all the tumor cells obtained from cows with clinical leukosis. This finding suggested that the possibility to use the MAb for diagnosis of EBL.7. Infectious pancreatic necrosis: Some of MAb-producing cells were produced. However, maintenance of the cells were failed; because of inability of cell passage or bacterial contamination.