A New Method for Determination of ABO Blood Groups using Nitrocellulose

（1） ABO小组使用ABC方法或混合的coll agglutination反应携带了指纹，并使用了Nitrocellulose膜。指纹的特征是通过轻轻按压手指上的硝酸纤维素膜通过实验性地准备的玻璃幻灯片在硝酸纤维素元件上的玻璃幻灯片被捕获的。1：混合细胞胶凝反应是通过培养反A、反B血清或反HULEC的膜膜，通过洗涤和进一步孵化，与A. B或O型细胞。这种技术相对来说是一种简化和清晰的方法，比使用贴纸胶片板的传统方法更有用，而且在法医科学领域进行血印的做法。2：以上的特性对反A和反B序列不同的干扰敏感化。具体地，吸收的抗体是用ABC方法可视化的。在结果中。A组和AB组指纹显示了反A whercas B组的积极站立 ... More 哦，哦，手指印，没有积极的表现。在类似的标记，B组和AB指纹显示出正面的反B标记。在A组和O组的指纹显示没有积极的表现。在这个实验中，任何积极的表现都不可能被观察到。（2）我们评估了使用硝酸纤维素珠子作为ABH血液组免疫吸附剂的优势。血液组物质在血液中被稀释和高度稀释的血液标本被吸收在船上。在哪种血型被混合细胞老化或被吸收-吸收技术确定之后。发现的老鼠比以前更敏感。当我们在25 ° C时植入1毫克硝酸纤维素珠子和10毫升血样，在1小时内装入ABH物质。A100，000-折叠出A型或B型的稀释抗原或50，000-折叠出的稀释抗原在这些条件下被检测到。没有提取物的程序。净化。或者说，antigen是needel的概念。有人建议，根据新方法描述，将在实践中找到医学-法律测试的痕迹证据中的应用。Less（低）

(1) ABO grouping was carried out on fingerprints lifted onto nitrocellulose membrane using the ABC method or mixed coll agglutination reaction. Specimens of fingerprints were obtained by gentle pressing of fingertips onto nitrocellulose membrenes by lifting the fingerprints experimentally prepared on glass slides onto nitrocellulose sembrenes .1:Mixed cell agglutination reaction was performed by incubating the membranes with anti-A, anti-B serum or anti-HUlec lectin followed by washing and further incubation with A.B or O type erythrocytes. This technic is relatively simpler and clearer rather than the conventional method using sticking film plate and is very useful in the practice of blood lyping of fingerprints in the forensic science field. 2:Above specimens were sensitized with anti-A and anti-B sera os varying dilutions. The specifically absorbed antibodies were visualized by the ABC method. In result. group A and AB fingerprints showed positive staning for anti-A whercas group B … More and O fingerprints ahowed no positive staining. in a similar manner, group B and AB fingerprints showed positive staining for anti-B. whereas group A and O fingerprints showed no positive staining. Any positive staining with appreciable internsity could not be observed in this experiment.(2) We evaluated the advantage of using nitrocellulose beads as immunoadsorbents for ABH blood groups. The blood group substances in hemolyzed and highly diluted blood specimens were absorbed on the boads. after which the blood type was determined either by mixed cell agglutination or by the absorption-elusion technique. The latter was found to be more sensitive than the former. An optimal absorption of the ABH substances was obtained when we incubated 1 mg nitrocellulose beads with 10 ml of the blood samples for 1 hour at 25゜C. A 100000-fold diluted antigen of the A or B type or a 50000-fold diluted antigen of the H type was detected under these conditions. No procedures of extraction. purification. or concentrations of the antigen were needel. It is suggested that the new methods described above will find wider application in the practice of medico-legal examination of trace evidence. Less