Production of specific pathogen-free(SPF)guinea-pigs and SPF rabbits by using gnotobiotes

SPF兔和SPF豚鼠的肠道植物群的控制比SPF小鼠和大鼠的肠道更重要，因为肠道植物群较差的无菌（GF）或前GF兔的盲肠尺寸变得非常大，并且它们对肠道感染敏感。用6株拟杆菌（B株）、B株与2株链球菌（B+S）接种无菌（GF）家兔，用常规（CV）家兔或接种CV家兔盲肠内容物（CF）的ex-GF小鼠盲肠内容物10倍稀释液培养植物群<-5>，和氯仿处理的CV兔（CHF）盲肠内容物。GF兔出生后3周经口接种B株或B+S株，第1天后3天接种CF或CHF。接种B菌株和CF的Ex-GF兔的存活率超过90%，并且它们的体重增加与CV兔相似。相反，仅接种CF或仅接种B菌株的ex-GF家兔在接种后3周内全部死亡。B+S组和CHF组的死亡率分别为50%和20%。但是，CV兔CF与ex-GF小鼠CF对GF兔的增重和健康状况均不理想，CV兔CF与ex-GF小鼠CF对GF兔的作用无差异。CV豚鼠的接种和CF可用于建立SPF群体的ex-GF豚鼠。

Control of the intestinal flora of SPF rabbits and SPF guineapigs are more important than that of SPF mice and rats, because the cecal size of germfree(GF) or ex-GF rabbits with poor intestinal flora become very large, and they are sensitive against enteric infections. In this study, we attempted to produce ex-GF rabbits and ex-GF guineapigs, and to establish SPF colonies.Germfree(GF) rabbits were inoculated with 6 strains of Bacteroides(B strains), B strains with 2 strains of Streptococcus(B+S), cultured flora from 10^<-5> dilution of cecal contents(CF) from a conventional(CV) rabbit or ex-GF mice inoculated with CF of CV rabbit, and chloroform-treated cecal contents of CV rabbit(CHF). GF rabbits were orally inoculated with B strains or B+S 3 weeks after birth, and then they were inoculated with CF or CHF 3 days after 1st. inoculation.Ex-GF rabbits inoculated with B strains and CF were survived at the rate of more than 90% and an increase of their body weight were similar to CV rabbits. On the contrary, all of ex-GF rabbits inoculated with only CF or with only B strains were dead within 3 weeks after inoculation. In B+S and CHF inoculated groups, mortality rate were 50% and 20%, respectively. However, weight gain and health condition of them were unsatisfactory, there were no differences on the effects to GF rabbits between CF from a CV rabbit and CF from ex-GF mice inoculated with CF from a CV rabbit.In case of guinea-pigs, the combination of Bacillus or Bifidobacterium as 1st. inoculation and CF from a CV guinea-pig was available for an establishment of ex-GF guinea-pigs for a SPF colony.