Development of gnotobiote mice for cancer research

开发用于癌症研究的悉生物小鼠

• 批准号: 05304059
• 负责人: MITSUOKA Tomotari
• 金额: $ 13.25万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05304059/
• 关键词: Gnotobiote; C-Ha-ras; DMH; Colon cancer; Bile acids; Transgenic mice; トランスジェニック マウス; 発ガン; Clostridium paraputriticum

Gnotobiotic transgenic mice with human cancer gene, c-Ha-ras gene, were Produced by oral inoculation with various intestinal bacteria isolated from human and mouse feces. We compared the colon tumor incidence after subcutaneous injection with dymetilhydrazin among various types of gnotobiotic mice. The tumor incidence of transgenic mice was higher than control mice in each group. The differences of floral composition affected the tumor incidence. In some cases floral bacteria stimulated the formation of tumors and in some cases inhibited. there were no relationship among tumor incidence, beta-glucuronidase activity and production of secondary bile acids.

用从人和小鼠粪便中分离的多种肠道细菌经口接种，制备了携带人癌基因c-Ha-ras的转基因小鼠。我们比较了不同类型的非细菌性小鼠皮下注射苯甲肼后结肠肿瘤的发生率。各组转基因小鼠的肿瘤发生率均高于对照组。花区组成的差异影响肿瘤的发生。在某些情况下，花卉细菌刺激肿瘤的形成，在某些情况下抑制。肿瘤发生率、β-葡萄糖醛酸酶活性和次级胆汁酸的产生之间没有关系。

项目成果

光岡知足: "腸内フローラと発癌" 学会出版センター, 176 (1995)

光冈智族：《肠道菌群与癌变》学会出版中心，176（1995）

Hirayama, K., Itoh, K., Mitsuoka, T.他: "Comparison of composition of faecal microbiota and metabolism of faecal bactosia among ‘Human-Floru-Associeted' mice with faeces……" Microbiol. Ecol. in Health & Dis.8. 199-211 (1995)

Hirayama, K.、Itoh, K.、Mitsuoka, T. 等人：“‘人类-弗洛鲁相关’小鼠与粪便中粪便微生物群的组成和粪便细菌代谢的比较……”《微生物学》。健康与疾病 .8。199-211 (1995)

Hirayama K.,Itoh K.,Mitsuoka T.他: "Development of intestinal flora of Human-Flora-Associated (HFA) mice in the intestine of their offspring" Exp.Anim.44. 219-222 (1995)

Hirayama K.、Itoh K.、Mitsuoka T. 等人：“人类菌群相关 (HFA) 小鼠后代肠道菌群的发育”Exp.Anim.44 (1995)。

Goto, K.Itoh, T.et al.: "Detection of Cilia-associated respiratory Bacillus in experimentally and naturally infected mice and rats by the polymerase chain reaction" Exp.Anim.44. 333-336 (1995)

Goto, K.Itoh, T.et al.：“通过聚合酶链反应检测实验和自然感染的小鼠和大鼠中纤毛相关呼吸道芽孢杆菌”Exp.Anim.44。

Hirayama,K.他: "Effects of dietary supplements on the composition of fecal flora of hnman-flora-associated(KFA)mice." Bifidobacteria Microflora. 13. 1-7 (1994)

Hirayama, K. 等人：“膳食补充剂对双歧杆菌相关 (KFA) 小鼠粪便菌群组成的影响。”13. 1-7 (1994)