Defining predictors of anti-viral immunity in the yellow fever vaccination model using gene polymorphisms and early innate response patterns (B14)

多个核酸传感器和抗原呈递细胞（APC）亚群的合作被认为对于产生针对感染和疫苗接种的有效且持久的适应性免疫至关重要。我们使用减毒活黄热病毒（YF17D）疫苗接种作为人类急性自限性RNA病毒感染的模型。我们将在YF17D疫苗接种前后对循环先天免疫细胞进行（表达）数量性状基因座（eQTL）分析和单细胞转录组和表观基因组分析。因此，我们将确定新的因素，调节核酸传感途径和破译抗病毒免疫的个体间变异的遗传基础。

Cooperation of multiple nucleic acid sensors and antigen presenting cell (APC) subpopulations is thought to be critical for generating effective and long-lasting adaptive immunity to infection and vaccination. We use the live-attenuated yellow fever virus (YF17D) vaccination as a model of an acute self-limiting RNA virus infection in humans. We will apply (expression) quantitative trait loci (eQTL) analysis and single cell transciptome and epigenome analysis of circulating innate immune cells before and after YF17D vaccination. Thereby we will identify new factors that regulate nucleic acid sensing pathways and decipher the genetic basis of inter-individual variability in antiviral immunity.