Elucidating the circuits of functional lateralization in the hippocampus

该项目的第一阶段是表征Gi-DREADD和KOR-DREADD在海马中的病毒表达的抑制作用和时间过程，并研究它们对应的配体对体内海马活动的影响。第二阶段通过行为任务研究左、右CA3区域在空间导航和记忆中的不同功能。在物体定位测试中，用两个熟悉的物体训练小鼠，在第二次回忆期间，在左侧CA3而不是右侧CA3沉默后，其中一个物体被移动到不同的位置。老鼠花了更多的时间在新位置探索物体，这归因于它们对新奇事物的天生偏好。这些发现表明，左CA3区域对空间记忆的获得更为重要。

The first stage of the project involved characterizing the inhibitory effects and time course of viral expression of Gi-DREADD and KOR-DREADD in the hippocampus, as well as examining their corresponding ligand effects on in vivo hippocampal activity.For the second stage, behavioral tasks were conducted to investigate the different functions of the left and right CA3 regions in spatial navigation and memory. In the object location test, mice were trained with two familiar objects, and during the second session of recall, one of the objects was moved to a different location after the left CA3 was silenced instead of the right CA3. The mice spent more time exploring the object in the novel location, which is attributed to their innate preference for novelty. These findings suggest that the left CA3 region is more crucial for the acquisition of spatial memory.